Thermo Fisher Scientific CD304 (Neuropilin-1) Monoclonal Antibody (TNKUSOHA), Alexa Fluor 700, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.125 µg)/test

Product Specifications

Available Formats

• APC-eFluor 780
• eFluor 450
• PE-eFluor 610
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

The TNKUSOHA monoclonal antibody recognizes human CD304 (also known as BDCA-4 and Neuropilin-1). CD304 is expressed on plasmacytoid dendritic cells in normal blood and bone marrow, as well as on T follicular helper cells in inflamed tonsils and some B-cell acute lymphoblastic leukemias. It is also found on non-hematopoietic cells including neurons, endothelial, and tumor cells.

Plasmacytoid dendritic cells play a key role in the innate immune response against viral pathogens and tumors.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-titrated and validated for flow cytometry using normal human peripheral blood cells
• Recommended Usage: 5 µL (0.125 µg) per test (defined as staining a cell sample in a final volume of 100 µL; 10⁵–10⁸ cells/test recommended)

Fluorochrome Characteristics:

• Alexa Fluor® 700 emits at 723 nm and is excited by the red laser (633 nm).
• Recommended filter setup: 685 LP mirror and 710/20 filter.
• Excitation: 633–647 nm; Emission: 723 nm; Laser: Red Laser.
• Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Neuropilin-1 is a 923 amino acid membrane-bound coreceptor belonging to the neuropilin family. It contains two CUB domains, two F5/8 type C domains, and a MAM domain. Neuropilin-1 forms heterodimers with NRP2 and binds plexin (PLXNB1) to form semaphorin receptors.

It is primarily expressed in the nervous system and is involved in:

• Cardiovascular system development
• Angiogenesis
• Axon guidance and neuronal circuit formation
• Cell survival and migration
• Organogenesis outside the nervous system

Neuropilin-1 also functions as a receptor for semaphorins and VEGF, regulating VEGF-induced angiogenesis. It may play a role in advanced prostate carcinoma and breast cancer progression.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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