Invitrogen™

Dynabeads™ Human DC Enrichment Kit

Use this kit to enrich viable human dendritic cells (DC`s) from PBMC by negative isolation. The kit depletes T cells,자세히 알아보기

Use this kit to enrich viable human dendritic cells (DCs) from PBMC by negative isolation. The kit depletes T cells, B cells, monocytes/ macrophages, granulocytes, NK cells and erythrocytes. Highly enriched DCs are bead and antibody-free, and can be used in any functional assay. The cells should be further sorted to obtain high purity of the specific DC sub-populations of choice.

• Obtain high recovery (80-100%) of viable DC`s of all sup-populations.
• Substantial reduction in sorting time and volume compared to sorting only, thus saving money as well.

Methodology:
An antibody mix towards the non- dendritic cells is added to the sample and allowed to bind to the cells. Dynabeads™ are added and will bind to the antibody-labeled cells during a short incubation. The bead-bound cells are quickly separated on a magnet and discarded. The remaining untouched human DC`s can be directly sorted into the sup-population of choice or used in any other application.

Starting samples:
PBMC

사양

세포 유형Dendritic Cells

제품 유형Cell Enrichment Kit

분리 기술Negative Isolation

셀 수Processes ∼2 x 109 cells total

출력 생존력>95%

제품라인DYNAL™, Dynabeads™

순도 또는 품질 등급Research Grade

수량2 x 10 mL

샘플 종류PBMC

배송 조건Room Temperature

시작 물질 셀 수1 x 107 PBMCs per isolation

타겟 종Human

Unit SizeEach