Thermo Fisher Scientific DES® TOPO® TA Expression Kit *DISCONTINUED*
PCR 산물을 DES® 발현 벡터에 직접 클로닝할 수 있는 원스텝 TA 클로닝 키트로, 빠르고 효율적인 재조합체 생산이 가능. pMT/V5-His-TOPO® 벡터를 사용하여 Drosophila S2 세포에서 단백질 발현, 검출, 정제를 간소화. 현재 단종된 제품.
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Thermo Fisher Scientific DES® TOPO® TA Expression Kit DISCONTINUED
The DES® TOPO® TA Expression Kit offers one-step cloning of PCR products directly into an inducible DES® expression vector.
This kit utilizes the linearized, topoisomerase I-activated pMT/V5-His-TOPO® vector for fast and easy cloning and subsequent high-level expression.
Topoisomerase activation of this vector allows PCR products to be ligated in just 5 minutes on your bench top, producing over 85% recombinants.
The pMT/V5-His-TOPO® vector includes all the features of the pMT/V5-His vector, simplifying protein expression, detection, and purification of recombinant proteins in Drosophila S2 cells.
사양 (Specifications)
| 항목 | 내용 |
|---|---|
| Unit Size | 40 reactions |
| Status | Discontinued |
| Vector | pMT/V5-His-TOPO® |
| Host System | Drosophila S2 cells |
| Cloning Type | TA cloning with topoisomerase I activation |
| Application | Inducible protein expression, detection, purification |
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