Thermo Fisher Scientific NEIL3 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide: L(164) R A E S E V K K Q K G R M L G(177) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:NEIL3,uniProtId:Q8TAT5-1,ncbiNodeId:9606,antigenRange:164-177,antigenLength:605,antigenImageFileName:PA1-4304_NEIL3_Q8TAT5-1_Rabbit.svg,antigenImageFileNamePDP:PA1-4304_NEIL3_Q8TAT5-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2298310

Product Specific Information

PA1-4304 detects the human NEI3 protein in human samples.

PA1-4304 has been successfully used in Western blot procedures. By Western blot this antibody detects a ~68 kDa protein representing NEI3 in human testis lysate.

PA1-4304 immunizing peptide corresponds to amino acid residues 164-177 of the human NEI3 protein. There is 85% sequence identity to mouse and rat.

Target Information

DNA glycosylase which prefers single-stranded DNA (ssDNA), or partially ssDNA structures such as bubble and fork structures, to double-stranded DNA (dsDNA). In vitro, displays strong glycosylase activity towards the hydantoin lesions spiroiminodihydantoin (Sp) and guanidinohydantoin (Gh) in both ssDNA and dsDNA; also recognizes FapyA, FapyG, 5-OHU, 5-OHC, 5-OHMH, Tg and 8-oxoA lesions in ssDNA. No activity on 8-oxoG detected. Also shows weak DNA-(apurinic or apyrimidinic site) lyase activity. In vivo, appears to be the primary enzyme involved in removing Sp and Gh from ssDNA in neonatal tissues. Seems to be an important facilitator of cell proliferation in certain populations, for example neural stem/progenitor cells and tumor cells, suggesting a role in replication-associated DNA repair.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.