Himedia RPMI-1640 w/ 1mM Sodium pyruvate, 2mM L-Glutamine, 4.5 gms Glucose per litre, 10mM HEPES buffer and 2 gms per litre Sodium bicarbonate

RPMI-1640 w/ 1mM Sodium pyruvate, 2mM L-Glutamine, 4.5 gms Glucose per litre, 10mM HEPES buffer and 2 gms per litre Sodium bicarbonate

Details

RPMI 1640 is the most commonly used medium in the Roswell Park Memorial Institute(RPMI) series developed by Moore et al for the culture of human normal and neoplastic cells in vitro. Its a modification of McCoys 5A medium specifically designed to support the growth of human lymphoblastoid cells in suspension culture. Presently the medium is extensively used for a wide range of anchorage dependant cell lines. The medium is known to support growth of cells both in presence of 5-20% FBS and in absence of FBS. AL162A is modified RPMI-1640 medium supplemented with L-glutamine, 4.5gms glucose, 10mM HEPES buffer, sodium pyruvate and sodium bicarbonate.