These kits are designed to quickly amplify segments of the rDNA region in either bacteria or fungi. The kits include a modified Taq DNA polymerase that allows rapid PCR amplification and primers for sequencing the PCR amplification products. In addition, the hot-start enzyme includes an anti-Taq antibody to prevent nonspecific amplification that results from pre-cycle mispriming or primer-dimer formation. Sequencing information obtained with this kit can be compared with sequence databases to classify microorganisms based on DNA sequence homology.