Thermo Fisher Scientific KRT19 Monoclonal Antibody (OTI1F5), TrueMAB
Human KRT19 단백질(aa 240-390)을 항원으로 한 Mouse IgG1 단클론 항체. Western blot과 IHC(P)에서 검증됨. Lyophilized 형태로 제공되며 PBS buffer(8% trehalose) 포함. 세포 및 조직 내 Cytokeratin 19 검출에 적합.
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Applications
Western Blot (WB)
- Tested Dilution: 1:200–1:1,000
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:100
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1F5 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 240–390 of human KRT19 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
Cytokeratin 19 is a member of the intermediate filament protein family, showing biochemical diversity across human epithelial tissues with at least 20 distinct polypeptides.
Cytokeratins range from 40–68 kDa in molecular weight and pI values between 4.9–7.8. Expression patterns of cytokeratin subtypes are used for distinguishing epithelial malignancies and assessing differentiation stages.
Cytokeratin 19 is not expressed in hepatocytes, making it useful for identifying liver metastasis.
Cytokeratin antibodies aid in differential tumor diagnosis, cytopathology, and flow cytometry assays.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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