Thermo Fisher Scientific Shaker Polyclonal Antibody, Biotin
Shaker 단백질 N-말단에서 유래한 합성 펩타이드로 제작된 Biotin 결합 Rabbit Polyclonal Antibody. Western blot과 IHC(P)에서 사용 가능하며, 인간 및 초파리 반응성. 항원 친화 크로마토그래피로 정제되어 안정적인 액상 형태로 제공.
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Applications
Western Blot (WB)
- Tested Dilution: 0.1–1 µg/mL
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 2–10 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Fruit fly, Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide derived from N-terminus of Shaker protein |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with proprietary stabilizer |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Positive control: Shaker over-expressed HEK293
- Cellular location: Membrane
Target Information
The assemblage of specific ion channels and receptors at synaptic sites is crucial for signaling between pre- and post-synaptic cells.
DLG (Drosophila discs-large gene) is co-localized with Shaker K1 channels, which are clustered at glutamatergic synapses.
DLG-Shaker interactions are required in vivo for shaker clustering at the neuromuscular junction.
The Shaker channel protein, properly synthesized, glycosylated, folded, assembled, and delivered to the plasma membrane, is essential for potassium channel function.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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