Thermo Fisher Scientific Shaker Polyclonal Antibody, Biotin

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-1 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

2-10 µg/mL

-

Product Specifications

Species Reactivity

Fruit fly, Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide derived from N-terminus of Shaker protein if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Shaker,uniProtId:P08510-1,ncbiNodeId:7227,antigenRange:1,antigenLength:655,antigenImageFileName:602-89B_Shaker_P08510-1_Rabbit.svg,antigenImageFileNamePDP:602-89B_Shaker_P08510-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

0.5 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with proprietary stabilizer

Contains

0.01% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Positive control: Shaker over-expressed HEK293

Cellular location: Membrane.

Target Information

The assemblage of specific ion channels and receptors at synaptic sites is crucial for signaling between pre- and post-synaptic cells. DLG (Drosophila discs-large gene) is co-localized with Shaker K1 channels, which are clustered at glutamatergic synapses. DLG-Shaker interactions are required in vivo for shaker clustering at the neuromuscular junction. The channel protein Shaker which is properly synthesized, glycosylated, folded, assembled and delivered to the plasma membrane is very important for the potassium channel function.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.