Thermo Fisher Scientific p130 Polyclonal Antibody
Human RBL2(p130)을 인식하는 Rabbit Polyclonal Antibody로 WB, IHC, ICC/IF에 사용 가능. 항원 친화 크로마토그래피로 정제되었으며, 액상 형태로 제공. 세포 분열 조절 및 히스톤 메틸화 연구에 적합.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 | - |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human RBL2. Recombinant protein control fragment (Product #RP-91613). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2789846 |
Product Specific Information
Immunogen sequence:
IAGSPLTPRR VTEVRADTGG LGRSITSPTT LYDRYSSPPA STTRRRLFVE NDSPSDGGTP GRMPPQPLVN AVPVQNVSGE TVSVTPVPGQ TLVTMATATV TANNGQTVTI PVQGIANENG GITFFPVQV
Target Information
RBL2 (also known as p130) or Retinoblastoma-like protein 2 is a key regulator of cell division entry. It is directly involved in heterochromatin formation by maintaining overall chromatin structure, particularly constitutive heterochromatin, through stabilization of histone methylation.
RBL2 recruits and targets histone methyltransferases KMT5B and KMT5C, leading to epigenetic transcriptional repression. It controls histone H4 Lys20 trimethylation and likely acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters.
RBL2 serves as a potent inhibitor of E2F-mediated trans-activation and associates preferentially with E2F5.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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