Thermo Fisher Scientific MRPL12 Polyclonal Antibody
Thermo Fisher Scientific MRPL12 Polyclonal Antibody는 인간 MRPL12 단백질을 인식하는 토끼 IgG 폴리클로날 항체로, WB, IHC, ICC/IF, ELISA에 사용 가능. 미토콘드리아 단백질 합성 연구용으로 적합하며, 고순도 친화 크로마토그래피 정제 제품.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1–198 of human MRPL12 (NP_002940.2) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.83 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2914758 |
Product Specific Information
- Positive test controls: A-431, 22Rv1, A-549
- Subcellular localization: Mitochondrion
Immunogen sequence:
MLPAAARPLW GPCLGLRAAA FRLARRQVPC VCAVRHMRSS GHQRCEALAG APLDNAPKEY PPKIQQLVQD IASLTLLEIS DLNELLKKTL KIQDVGLVPM GGVMSGAVPA AAAQEAVEED IPIAKERTHF TVRLTEAKPV DKVKLIKEIK NYIQGINLVQ AKKLVESLPQ EIKANVAKAE AEKIKAALEA VGGTVVLE
Target Information
MRPL12 (mitochondrial ribosomal protein L12), also known as 5c5-2, L12mt, MRPL7, or RPML12, is a mammalian mitochondrial ribosomal protein involved in protein synthesis within the mitochondrion.
It is enhanced in growth-stimulated cells due to transcriptional activation, functioning as a translational regulator of mitochondrial mRNAs.
Impairment of MRPL12 leads to reduced cell growth rate, decreased mitochondrial ATP production, and loss of oxidative phosphorylation.
It is cleaved during translocation across the mitochondrial membrane and forms dimers that bind the large ribosomal subunit.
MRPL12 consists of 198 amino acids, belongs to the ribosomal protein L12P family, and is highly expressed in the colon.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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