Thermo Fisher Scientific V-ATPase H Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.04-0.4 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunocytochemistry (ICC/IF)

0.25-2 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human V-ATPase H. Recombinant protein control fragment (Product #RP-93733). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:V-ATPase H,uniProtId:Q9UI12-1,ncbiNodeId:9606,antigenRange:404-474,antigenLength:483,antigenImageFileName:PA5-54793_V-ATPase_H_Q9UI12-1_Rabbit.svg,antigenImageFileNamePDP:PA5-54793_V-ATPase_H_Q9UI12-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2649409

Product Specific Information

Immunogen sequence: PQVLAVAAHD VGEYVRHYPR GKRVIEQLGG KQLVMNHMHH EDQQVRYNAL LAVQKLMVHN WEYLGKQLQS E

Highest antigen sequence identity to the following orthologs: Mouse - 100%, Rat - 100%.

Target Information

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of intracellular organelles. V-ATPase-dependent organelle acidification is necessary for multiple processes including protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. The encoded protein is the regulatory H subunit of the V1 domain of V-ATPase, which is required for catalysis of ATP but not the assembly of V-ATPase. Decreased expression of this gene may play a role in the development of type 2 diabetes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.