Thermo Fisher Scientific UFD1L Monoclonal Antibody (OTI4C7), TrueMAB

Applications

Western Blot (WB)

• Tested Dilution: 1:2,000

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:150

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).

Target Information

Ubiquitin-mediated proteolysis involves transferring ubiquitin (Ub) to lysine groups on target proteins, leading to degradation by the 26S proteasome.
Ub-fusions are processed either by Ub-specific proteases (UBps) or via the Ub-fusion degradation (UFD) pathway.
Human UFD1L, homologous to yeast UFD1, modulates degradation of Ub-fusions with specific amino acid substitutions.
Mutations in the UFD1 gene are associated with CATCH22 syndrome, characterized by cardiac defects, cleft palate, and hypocalcemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.