Thermo Fisher Scientific H3K27ac Monoclonal Antibody (A6D7)
H3K27ac 단일클론 항체(A6D7)는 인간, 마우스, 랫트에 반응하며 다양한 응용(WB, IHC, ICC/IF, Flow, ChIP)에 적합합니다. 단일클론 Mouse IgG1 항체로, 고순도 Protein G 정제. 액상 형태로 안정적이며 4°C 또는 -20°C 보관 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:600 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:1,000 |
| ChIP assay (ChIP) | 0.5–2 µg |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | A6D7 |
| Immunogen | Synthetic peptide within human Histone H3 aa 20–40 (acetyl K27) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 2 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS, pH 7.4, with 40% glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2911686 |
Product Specific Information
- Positive Controls: 293 cell lysate, MCF-7 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, HeLa cell lysate, human tonsil tissue
- Subcellular Location: Nucleus, Chromosome
Target Information
Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3, along with four core histone proteins, binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. Post-translationally, histones are modified in various ways to either directly change chromatin structure or allow for binding of specific transcription factors. The N-terminal tail of histone H3 protrudes from the nucleosome core and can undergo multiple post-translational modifications, such as methylation or acetylation of lysine and arginine residues, and phosphorylation of serine or threonine.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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