Thermo Fisher Scientific H3K27ac Monoclonal Antibody (A6D7)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:600

-

Immunocytochemistry (ICC/IF)

1:100

-

Flow Cytometry (Flow)

1:1,000

-

ChIP assay (ChIP)

0.5-2 µg

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

A6D7

Immunogen

Synthetic peptide within human Histone H3 aa 20-40 (acetyl K27) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:H3K27ac,uniProtId:P68431-1,ncbiNodeId:9606,antigenRange:20-40,antigenLength:136,antigenImageFileName:MA5-42545_H3K27ac_P68431-1_House_mouse.svg,antigenImageFileNamePDP:MA5-42545_H3K27ac_P68431-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 40% glycerol, 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2911686

Product Specific Information

Positive Control: 293 cell lysate, MCF-7 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, Hela cell lysates, Hela, human tonsil tissue.

Subcellular Location: Nucleus, Chromosome.

Target Information

Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.