Thermo Fisher Scientific CEP135 Polyclonal Antibody

Applications

Western Blot (WB)

• Assay-dependent

Immunoprecipitation (IP)

• 5–10 µg/mg lysate

Product Specifications

Product Specific Information

• The recommended shelf life for this product is 1 year from the date of receipt.

Target Information

LATS1 is a putative serine/threonine kinase that localizes to the mitotic apparatus and complexes with cell cycle controller CDC2 kinase in early mitosis. The protein is phosphorylated in a cell-cycle dependent manner, with late prophase phosphorylation remaining through metaphase.
The N-terminal region binds CDC2 to form a complex showing reduced H1 histone kinase activity, indicating a role as a negative regulator of CDC2/cyclin A.
The C-terminal kinase domain binds to its own N-terminal region, suggesting potential negative regulation through intramolecular binding.
Biochemical and genetic data suggest a role as a tumor suppressor, supported by studies in knockout mice showing development of soft-tissue sarcomas, ovarian stromal cell tumors, and high sensitivity to carcinogenic treatments.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.