Applied Biosystems™

TaqMan™ Universal Master Mix II, with UNG

TaqMan Universal Master Mix II is a convenient mix of components (except primers, probes, template, and water) necessary to perform자세히 알아보기

TaqMan Universal Master Mix II is a convenient mix of components (except primers, probes, template, and water) necessary to perform a real-time polymerase chain reaction (PCR). Use TaqMan Universal Master Mix II to amplify complementary DNA (cDNA) and DNA targets for a variety of applications, including quantitation and genotyping.

This TaqMan Universal Master Mix II is supplied as a 2X concentrate and contains:
• AmpliTaq Gold DNA Polymerase, UP (Ultra Pure)
• dNTPs (with dUTP)
• ROX Passive Reference
• Uracil-N-glycosylase (UNG)
• Optimized buffer components

Note: This TaqMan Universal Master Mix II contains uracil-N-glycoslyase (UNG). See a complete listing of all TaqMan Universal Master Mix II products, with and without UNG.

TaqMan Universal Master Mix II has been optimized for use with primers and TaqMan probes that have been designed according to Thermo Fisher Scientific guidelines. The master mix can be used with custom TaqMan assays available from our custom assay service, or with pre-optimized assays such as:
• TaqMan gene expression assays
• TaqMan microRNA assays
• TaqMan drug metabolism genotyping assays
• TaqMan SNP genotyping assays

For RNA quantitation experiments, TaqMan Universal Master Mix II is used in the second step of a two-step reverse transcription/polymerase chain reaction (RT-PCR) protocol. The cDNA template used with the master mix can be generated in a reverse transcription reaction using cDNA synthesis kits available from Thermo Fisher Scientific.

Alternative product: Try TaqMan Fast Advanced Master Mix, our highest-performance, probe-based master mix. With TaqMan Fast Advanced Master Mix, we`ve taken the best of TaqMan Universal Master Mix II and added additional capabilities for your gene expression analysis.

사양

검출 방법Primer-probe

GC-Rich PCR PerformanceHigh

PCR 방법qPCR

중합효소AmpliTaq Gold DNA Polymerase

반응 속도Standard

용도(장비)7300 System, 7500 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System

패시브 참조 염료ROX (Pre-mixed)

제품라인TaqMan™

제품 유형Universal Master Mix II

순도 또는 품질 등급UP (Ultra Pure)

수량1 x 5 mL

배송 조건Wet Ice

용도(애플리케이션)Gene Expression, Genotyping, miRNA analysis

농도2X

Fidelity (Taq 대비)2 X

샘플 종류DNA (Genomic), cDNA

부피5 mL

Unit SizeEach