Thermo Fisher Scientific PNPase Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.04-0.4 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunocytochemistry (ICC/IF)

0.25-2 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human PNPT1. Recombinant protein control fragment (Product #RP-96093). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PNPase,uniProtId:Q8TCS8-1,ncbiNodeId:9606,antigenRange:647-741,antigenLength:783,antigenImageFileName:PA5-83236_PNPase_Q8TCS8-1_Rabbit.svg,antigenImageFileNamePDP:PA5-83236_PNPase_Q8TCS8-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.3 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2790392

Product Specific Information

Immunogen sequence: FSVFAPTPSA MHEARDFITE ICKDDQEQQL EFGAVYTATI TEIRDTGVMV KLYPNMTAVL LHNTQLDQRK IKHPTALGLE VGQEIQVKYF GRDPA

Target Information

PNPT1 (polyribonucleotide nucleotidyltransferase 1, mitochondirla) is a RNA-binding protein implicated in numerous RNA metabolic processes. It catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3-to-5 direction. PNPT1 is a component of the mitochondrial degradosome (mtEXO) complex that degrades 3overhang double-stranded RNA with a 3-to-5` directionality in an ATP-dependent manner. It is required for correct processing and polyadenylation of mitochondrial mRNAs. It plays a role as a cytoplasmic RNA import factor, in mitochondrial morphogenesis and respiration, regulation of expression of the electron transport chain, regulation of the stability of specific mature miRNAs in melanoma cells, and RNA cell surveilance. Mutations of the gene can result in combined oxidative phosphorylation deficiency 13 (COXPD13) and deafness, autosomal recessive, 70 (DFNB70).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.