Thermo Fisher Scientific CD72 Monoclonal Antibody (J4-117), eFluor 660, eBioscience

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.06 µg)/test

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2b, kappa

Recommended Isotype Control

Mouse IgG2b kappa Isotype Control (eBMG2b), eFluor™ 660, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

J4-117

Conjugate

eFluor™ 660 eFluor™ 660 eFluor™ 660

Excitation/Emission Max

651/668 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2574173

Product Specific Information

Description: The monoclonal antibody J4-117 reacts with human CD72, a 45 kDa type II transmembrane glycoprotein that belongs to the C-type lectin superfamily. This glycoprotein is expressed on B cells at all stages of maturation except for plasma cells. CD72 is also expressed on dendritic cells, mast cells, and some macrophages. This receptor contains an immune-receptor tyrosine-base inhibition motif (ITIM) that associates with a tyrosine phosphatase, SHP-1. CD72 negatively regulates the major signal transduction pathways activated by the B cell receptor (BCR). Studies have demonstrated that the ligand for CD72 is CD100 (also known as Semaphorin 4D). CD72 can also react with CD5 to provide signaling between T and B cells. Finally, this receptor also prevents naive B cells from producing Ig and differentiating into B cells, and plays a role in controlling autoimmunity by enhancing apoptosis after Ag-specific BCR stimulation.

Applications Reported: This J4-117 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This J4-117 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD72 is a transmembrane glycoprotein expressed as a homodimer especially in B cells, but also in other antigen presenting cells such as dendritic cells and macrophages. Through one of its immunoreceptor tyrosine-based inhibitory motives (ITIMs), CD72 interacts with tyrosine phosphatase SHP-1, thereby suppressing B cell responsiveness. Binding of CD72 with its ligand CD100 (Sema4D) prevents BCR association and phosphorylation of CD72 and results in dissociation of SHP-1 from CD72, thus enables B cell activation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.