Thermo Fisher Scientific Human AURKB (Aurora B), His Tag Recombinant Protein

Thermo Fisher Scientific Human AURKB (Aurora B), His Tag Recombinant Protein

Applications

• Kinase Assay (KA)

Tested Dilution: Assay-dependent
Publications: [References component placeholder]

Product Specifications

Product Specific Information

• For maximum recovery, please spin prior to use.
• Aliquots of 5 µg, 10 µg, and 20 µg sizes are not recommended; use original packaging until exhausted.
• For larger sizes, aliquot preparation can reduce freeze/thaw cycles; aliquots below 20 µL are not recommended.
• Do not store kinase diluted.
• Proper storage at -80°C ensures product stability for 6 months from date of purchase.

Protein Form: Recombinant, Full Length

Target Information

Aurora kinases are associated with microtubules during chromosome movement and segregation.
AURKB (Aurora B) localizes to microtubules near kinetochores, specifically to K-fibers, and functions in:

• Chromosome alignment and separation
• Histone modification
• Cytokinesis

The Chromosomal Passenger Complex (CPC) includes:

• INCENP (Inner Centromere Protein)
• Survivin
• Borealin

These subunits regulate Aurora B’s activity, localization, stability, and substrate specificity.
During mitosis, two CPC forms exist:

1. Full complex (AurB + INCENP + Survivin + Borealin)
2. INCENP-AurB complex

AurB is overexpressed in several tumors, including breast and colon cancers, and may interact with Aurora-A kinase in a feedback mechanism.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.