Thermo Fisher Scientific KAP-1 Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:2,000–1:10,000

Immunoprecipitation (IP)

• Tested Dilution: 2–10 µg/mg lysate

Product Specifications

Product Specific Information

• Recommended shelf life: 1 year from date of receipt
• Based on 100% sequence identity, this antibody is predicted to react with Rat

Target Information

The KRAB (Kruppel-associated box) domain is approximately 45 amino acids long and functions as a transcriptional repression domain found in many transcription factors. Over 220 KRAB-zinc finger protein (KRAB-ZFP) genes have been identified in the human genome. These proteins repress transcription through interactions with KAP-1 (KRAB-associated protein 1).
KAP-1 is an 835 amino acid protein containing a RING finger, B boxes, and a PHD finger. It forms complexes with KRAB-domain transcription factors to enhance transcriptional repression efficiency. KAP-1 also interacts directly with HP1 (heterochromatin protein 1) and KRAZ1 (Kruppel-associated box-containing zinc finger protein 1), targeting KRAZ1 to centromeric heterochromatin foci containing HP1α, thereby regulating transcriptional repression.
Mutant KAP-1 proteins that bind KRAB but not HP1 cause random KRAZ1 distribution and strong transcriptional activation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.