Thermo Fisher Scientific PBK Polyclonal Antibody
PBK 단백질을 인식하는 Rabbit Polyclonal Antibody로, WB, IHC, IP 등 다양한 실험에 사용 가능. 항원 친화 크로마토그래피로 정제되었으며, 1 mg/mL 농도의 액상 형태. Human 시료에 반응하며, 4°C에서 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 | |
| Immunohistochemistry (IHC) | 1:500–1:2,000 | |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:2,000 | |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 200 and 250 of human PDZ binding kinase (PBK). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Tris citrate/phosphate, pH 7–8 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Target Information
PBK (TOPK) is a serine/threonine kinase related to the dual specific mitogen-activated protein kinase (MAPKK).
It is up-regulated in various hematological malignancies, participates in lymphoid cell activation, and supports testicular function (spermatogenesis).
Mitotic phosphorylation is required for its catalytic activity, suggesting a role in cell division and spermatogenesis.
For Research Use Only. Not for use in diagnostic procedures.
Not for resale without express authorization.
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