Thermo Fisher Scientific CNOT4 Polyclonal Antibody
Rabbit polyclonal antibody targeting human CNOT4 protein. Suitable for Western blot and IHC (Paraffin). Purified by antigen affinity chromatography. Supplied as liquid form, unconjugated, with 0.1 mg/mL concentration. For research use only.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human CNOT4. Recombinant protein control fragment (Product #RP-102476). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2789375 |
Product Specific Information
Immunogen sequence:
TESQSLFSDN FRHPNPIPSG LPPFPSSPQT SSDWPTAPEP QSLFTSETIP VSSSTDWQAA FGFGSSKQPE DDLGFDPFDV TRKALADLIE KELSVQDQPS LSPTSLQNSS SHTTTAKGPG SGFLHPAAAT NANSLNSTFS VLPQRF
Target Information
CNOT4 is a 575 amino acid protein with E3 ubiquitin ligase activity, mainly involved in protein modification and ubiquitination.
It contains a C4C4-type zinc finger domain, an RNA recognition motif, and a bipartite nuclear localization signal.
CNOT4 is a component of the CCR4-NOT (CAF1) core complex, functioning as a general transcription regulation complex with both positive and negative effects on transcription of various genes.
It interacts with CNOT1 via its C-terminus and with E2 ubiquitin ligases via its C4C4 RING domain.
Reports suggest CNOT4 is phosphorylated in response to DNA damage at consensus sites recognized by ATM and ATR.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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