Thermo Fisher Scientific Cas9 Monoclonal Antibody (7A9)
CRISPR Cas9 단백질 검출용 Thermo Fisher Scientific의 단일클론 항체(7A9). Western blot, IHC, ICC, IP 등 다양한 응용에 적합. Streptococcus pyogenes 유래 Cas9 인식. 고순도 Protein A 정제, 2 mg/mL 농도, 안정적 PBS buffer 보관.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000–1:6,000 | View 3 publications |
| Immunohistochemistry (IHC) | – | View 1 publication |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | – | View 1 publication |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 | View 1 publication |
| Immunoprecipitation (IP) | 1:200 | – |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Bacteria |
| Published Species | Bacteria, Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 7A9 |
| Immunogen | Cas9 nuclease (CRISPR-associated protein 9) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 2 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2610639 |
Product Specific Information
MA5-23519 is derived from Streptococcus pyogenes.
Target Information
Cas9 is an RNA-guided DNA endonuclease enzyme associated with the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) adaptive immunity system in bacteria and genetic engineering.
This system uses RNA to guide the nuclease to a specific DNA sequence, enabling targeted genome editing such as indel mutations, sequence insertions, deletions, or rearrangements.
Cas9 can also be used for:
- Upregulation of endogenous genes
- Alteration of histone modifications
- DNA methylation changes
Cas9 variants include:
- SpCas9 (Streptococcus pyogenes Cas9)
- SaCas9 (Staphylococcus aureus Cas9)
SaCas9 advantages:
- Smaller size (1053 AA vs. 1368 AA for SpCas9)
- High efficiency and specificity
- Nickase activity
- Recognizes NNGRRT PAM
- Suitable for all-in-one delivery systems with AAV vectors
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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