Applied Biosystems™

TaqMan™ Pri-miRNA Assay

TaqMan Pri-miRNA Assays quantitate primary microRNA (pri-miRNA) transcripts, the most upstream RNA molecules in the microRNA biogenesis pathway. They are자세히 알아보기

TaqMan Pri-miRNA Assays quantitate primary microRNA (pri-miRNA) transcripts, the most upstream RNA molecules in the microRNA biogenesis pathway. They are designed using our state-of-the-art bioinformatics pipeline, delivering gold-standard assay performance and data quality for pri-miRNA studies.

• Highly specific—quantitiate miRNA transcription from a single genomic locus
• Fast, simple, and scalable—two-step RT-qPCR provides high-quality results
• A new perspective—measure miRNA expression at the gene level

TaqMan Pri-miRNA Assays apply the same high sensitivity, superior specificity, and wide linear dynamic range for which all TaqMan genomic assays are renowned to the detection of pri-miRNA transcripts. As a result of their high sensitivity, TaqMan Pri-miRNA Assays require as little as 1 ng of sample input to quantify expression from moderate- to high-expression miRNA loci.

Design and selection
Since all pri-miRNA transcripts have not been mapped, these assays are designed within close proximity to each stem-loop sequence represented in the Sanger miRBase sequence repository. This ensures accurate measurement of the transcript of interest. Each publicly available stem-loop can be matched to a TaqMan Pri-miRNA Assay and TaqMan MicroRNA Assay, enabling the RNA sequences produced at either end of the miRNA maturation pathway to be quantified independently.

Pre-formulated assay (20X; 60X mix)
• 2 unlabeled PCR primers (900 nM each final 1X reaction concentration)
• 1 FAM dye-labeled TaqMan MGB probe (250 nM final 1X reaction concentration)

사양

형식Tube

GC-Rich PCR PerformanceHigh

PCR 방법qPCR

반응 속도Fast

그린 기능Sustainable packaging

제품라인TaqMan™

제품 유형Pri-miRNA Assay

수량S (360 reactions), made to order

배송 조건Room Temperature

충분360 Reactions

농도20X

Fidelity (Taq 대비)60 X

반응 수360 reactions

Unit SizeEach