Thermo Fisher Scientific KT3 Polyclonal Antibody
KT3 태그 단백질 검출용 Thermo Fisher Scientific의 폴리클로날 항체. WB, ICC/IF, ELISA, IP 등 다양한 응용에 사용 가능. 염소 유래 비결합형 항체로, 1 mg/mL 농도 및 PBS 버퍼에 보관. 연구용으로 세포 신호전달 및 단백질 분석에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:20,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:400 |
| ELISA | 1:1,000–1:30,000 |
| Immunoprecipitation (IP) | 1–4 µg/mg |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host/Isotype | Goat |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KT3 (KPPTPPPEPET) conjugated to KLH |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
The KT3 tag does not correspond to a specific gene but is commonly used in biochemical and molecular biology research as a peptide tag for protein detection and purification.
It is related to the PI3K/AKT/mTOR signaling pathway, a key regulatory network in eukaryotic cells involved in cell survival, growth, and metabolism.
PI3K (phosphatidylinositol 3-kinase) generates phosphatidylinositol-3,4,5-trisphosphate (PI(3,4,5)P3), which acts as a second messenger facilitating Akt activation at the plasma membrane.
This pathway is frequently activated in various cancers, contributing to tumorigenesis and cancer progression by promoting cell growth and inhibiting apoptosis.
Targeted therapies inhibiting this pathway are under investigation for potential cancer treatment.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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