Thermo Fisher Scientific ATP Citrate Lyase Monoclonal Antibody (5F8D11)
ATP Citrate Lyase 단백질을 검출하는 Mouse 모노클로날 항체로, WB, IHC, ICC/IF, Flow Cytometry, ELISA에 사용 가능. Human, Mouse, Rat 등 다양한 종에 반응하며, Protein G 정제 및 PBS buffer에 보관. 연구용으로 적합.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | View 2 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:50 | - |
| Flow Cytometry (Flow) | 1:200–1:400 | - |
| ELISA | 1:10,000 | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 5F8D11 |
| Immunogen | Purified recombinant fragment of human ACLY (amino acids 306–502) expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2538499 |
Product Specific Information
MA5-17027 targets ACLY in indirect ELISA, FACS, ICC, IHC, IF, and WB applications and shows reactivity with Human, Mouse, Non-human primate, and Rat samples.
The immunogen is a purified recombinant fragment of human ACLY (amino acids 306–502) expressed in E. coli.
MA5-17027 detects ACLY, which has a predicted molecular weight of approximately 125 kDa.
Target Information
ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (molecular weight ≈ 440,000) composed of identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA, with concomitant hydrolysis of ATP to ADP and phosphate.
Acetyl-CoA serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate lyase may be involved in acetylcholine biosynthesis.
Two transcript variants encoding distinct isoforms have been identified for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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