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Thermo Fisher Scientific CD301b (MGL2) Monoclonal Antibody (11A10-B7), eFluor 660, eBioscience

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Mouse CD301b (MGL2) 인식하는 단클론 항체로, eFluor 660 형광 표지. Flow cytometry에 최적화되어 있으며, 0.25 µg/test 농도로 사용 가능. 피부 및 골수 유래 수지상세포 연구에 적합. 4°C 암조건 보관, 동결 금지.

카탈로그번호: 50-3011-82
브랜드: Thermo Fisher Scientific
카테고리: Monoclonal Antibodies
판매단위: pk

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Thermo Fisher Scientific 50-3011-82 CD301b (MGL2) Monoclonal Antibody (11A10-B7), eFluor 660, eBioscience 100 ug pk판매 단위 pk ·

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540,600원VAT 포함 594,660원

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Thermo Fisher Scientific · Thermo Fisher Scientific CD301b (MGL2) Monoclonal Antibody (11A10-B7), eFluor 660, eBioscience

Thermo Fisher Scientific CD301b (MGL2) Monoclonal Antibody (11A10-B7), eFluor 660

Applications and Tested Dilution

Application	Tested Dilution	Publications
Immunohistochemistry (IHC)	-	View 1 publication
Immunohistochemistry (Frozen) (IHC (F))	-	View 1 publication
Flow Cytometry (Flow)	0.25 µg/test	View publication

Product Specifications

항목	내용
Species Reactivity	Mouse
Published Species	Mouse
Host / Isotype	Rat / IgG2a, lambda
Recommended Isotype Control	Rat IgG2a kappa Isotype Control (eBR2a), eFluor™ 660, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	11A10-B7
Conjugate	eFluor™ 660
Excitation/Emission Max	651 / 668 nm
Form	Liquid
Concentration	0.2 mg/mL
Purification	Affinity chromatography
Storage Buffer	PBS, pH 7.2
Contains	0.09% sodium azide
Storage Conditions	4°C, store in dark, DO NOT FREEZE
Shipping Conditions	Ambient (domestic); Wet ice (international)
RRID	AB_2574197

Additional Formats

Product Specific Information

The monoclonal antibody 11A10-B7 recognizes mouse CD301b (macrophage galactose-type C-type lectin 2, MGL2). CD301b expression is restricted to dermal dendritic cells, connective tissue, and bone marrow-derived dendritic cells. Macrophages also express CD301b at low levels.

CD301b contains a carbohydrate recognition domain that binds protein antigens with galactose and N-acetylgalactosamine (Gal/GalNAc). These antigens are internalized via CD301b and presented to CD4+ T cells through MHC Class II. CD301b+ dendritic cells play an important role in TH2 priming.

Applications Reported: Flow cytometric analysis.
Applications Tested: Tested on mouse skin draining lymph node cells at ≤ 0.25 µg/test (100 µL final volume). Cell number may range from 10⁵–10⁸ cells/test. Optimize titration for best performance.

Fluorochrome Information:
eFluor® 660 is a replacement for Alexa Fluor® 647. Emits at 659 nm and is excited by the red laser (633 nm). Ensure instrument compatibility.
Excitation: 633–647 nm; Emission: 668 nm; Laser: Red.
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD301b (MGL2) is expressed mainly in dermal and bone marrow-derived dendritic cells. It mediates internalization of Gal/GalNAc-containing antigens and contributes to TH2 immune response priming.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.