ELK Biotechnology Histone H4 (Tri Methyl Lys21) rabbit pAb

Histone H4 (Tri Methyl Lys21) rabbit pAb

제품 정보

Background

Function:
Core component of the nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to cellular machineries requiring DNA as a template. Histones play a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. DNA accessibility is regulated via post-translational modifications of histones (histone code) and nucleosome remodeling.

Post-Translational Modifications (PTMs):

• Acetylation at Lys-6, Lys-9, Lys-13, and Lys-17 occurs in coding regions but not in heterochromatin.
• Citrullination at Arg-4 by PADI4 impairs methylation.
• Monomethylation, dimethylation, or trimethylation at Lys-21. Monomethylation by SET8; trimethylation by SUV420H1 and SUV420H2 induces gene silencing.
• Monomethylation at Arg-4 by PRMT1 favors acetylation at Lys-9 and Lys-13; demethylation by JMJD6.
• Sumoylation is associated with transcriptional repression.
• Ubiquitination by the CUL4-DDB-RBX1 complex in response to UV irradiation may weaken histone-DNA interaction and facilitate DNA accessibility for repair.

Similarity:
Belongs to the histone H4 family.

Subunit:
The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3, and H4, assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.