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ELK Biotechnology MMP1 (Cleaved-Ile271) rabbit pAb
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ELK Biotechnology MMP1 (Cleaved-Ile271) rabbit pAb

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Human MMP1(Cleaved-Ile271)에 특이적인 rabbit polyclonal antibody로 WB, ELISA, IHC에 사용 가능. 고순도 IgG, 1 mg/ml 농도. 세포외 기질 단백질 검출 및 콜라겐 분해 효소 연구에 적합. -20°C에서 1년 보관.

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pk
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ELK Biotechnology ES20022-100UL MMP1 (Cleaved-Ile271) rabbit pAb, 100UL pk판매 단위 pk ·
재고 확인 필요
402,000원VAT 포함 442,200원
ELK Biotechnology ES20022-50UL MMP1 (Cleaved-Ile271) rabbit pAb, 50UL pk판매 단위 pk ·
재고 확인 필요
301,000원VAT 포함 331,100원

ELK Biotechnology · ELK Biotechnology MMP1 (Cleaved-Ile271) rabbit pAb

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MMP1 (Cleaved-Ile271) rabbit pAb

기본 정보

항목 내용
대체명 (Alternative Names) Interstitial collagenase (EC 3.4.24.7); Fibroblast collagenase; Matrix metalloproteinase-1; MMP-1 [Cleaved into: 22 kDa interstitial collagenase; 27 kDa interstitial collagenase]
적용 분야 (Applications) WB; ELISA; IHC
권장 희석 배율 (Recommended Dilutions) WB 1:500–2000; IHC-p 1:50–300; ELISA 1:2000–20000
면역원 (Immunogen) Synthesized peptide derived from human MMP1 (Cleaved-Ile271)
종 (Host) Rabbit
보관 조건 (Storage) -20°C / 1 year
클론성 (Clonality) Polyclonal
아이소타입 (Isotype) IgG
농도 (Concentration) 1 mg/ml
관찰 밴드 (Observed Band) 22, 53 kD
Gene ID (Human) 4312
Human Swiss-Prot No. P03956
세포 내 위치 (Cellular Localization) Secreted, extracellular space, extracellular matrix
반응 종 (Species Reactivity) Human; Rat; Mouse

배경 (Background)

  • Catalytic activity: Cleaves the triple helix of collagen at approximately three-quarters of its length from the N-terminus (775-Gly-|Ile-776 in alpha-1(I) chain).
  • Substrate specificity: Cleaves collagens of types I, II, III, VII, and X.
  • Cofactors: Binds 2 zinc ions and 4 calcium ions per subunit.
  • Domain structure: Contains catalytic N-terminal and C-terminal domains; C-terminal domain binds TIMP (tissue inhibitor of metalloproteinases).
  • Regulation: Can be activated without removal of the activation peptide.
  • Function: Involved in extracellular matrix remodeling and collagen degradation; interacts with HIV-1 Tat protein, reducing Tat-mediated neurotoxicity.
  • Post-translational modification (PTM): Undergoes autolytic cleavage to major forms (22 kDa and 27 kDa); minor glycosylated form (25 kDa) derived from 22 kDa form.
  • Similarity: Belongs to the peptidase M10A family; contains 4 hemopexin-like domains.
  • Subunit interaction: Interacts with HIV-1 Tat.

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