Thermo Fisher Scientific NANOS3 Polyclonal Antibody
인간 NANOS3 단백질을 인식하는 Rabbit Polyclonal 항체로, IHC(P)에서 1:500–1:1000 희석 비율로 사용 가능. 항원 친화 크로마토그래피로 정제되었으며, 0.3 mg/mL 농도의 액상 형태. 생식세포 발달 연구용으로 적합하며, 연구용으로만 사용.
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Applications and Tested Dilution
- Application: Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:500–1:1000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human NANOS3. Recombinant protein control fragment (Product #RP-103481) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.3 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Wet ice |
| RRID | AB_2644462 |
Product Specific Information
Immunogen sequence:
LAHLVRALSG KEGPETRLSP QPEPEPMLEP VSALEPMPAP ESVPVPGPKD QKRSLESSPA PERLCSFCKH NG
Sequence identity:
- Mouse: 46%
- Rat: 46%
Target Information
Nanos is a zinc-finger containing, RNA-binding protein implicated in germ cell development in both invertebrates and vertebrates. In Drosophila, Nanos represses apoptosis during development to ensure proper germ-line development.
Unlike Nanos1, whose expression in mice is dispensable, Nanos2 and Nanos3 proteins are required for germ cell development.
- Nanos2− PGCs die only in male gonads and show no defects in females.
- Nanos3− PGCs are lost during the migration stage regardless of sex.
Nanos2 and Nanos3 have distinct expression patterns during embryonic development, suggesting non-redundant functions. However, expression of Nanos2 can partially replace Nanos3 function in a Nanos3− background, while Nanos3 expression cannot rescue Nanos2− defects.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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