Thermo Fisher Scientific NANOS3 Polyclonal Antibody

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

1:500-1:1000

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human NANOS3. Recombinant protein control fragment (Product #RP-103481).

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.3 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2644462

Product Specific Information

Immunogen sequence: LAHLVRALSG KEGPETRLSP QPEPEPMLEP VSALEPMPAP ESVPVPGPKD QKRSLESSPA PERLCSFCKH NG

Highest antigen sequence identity to the following orthologs: Mouse - 46%, Rat - 46%.

Target Information

Nanos is a zinc-finger containing, RNA-binding protein that has been implicated in germ cell development in both invertebrates and vertebrates. In Drosophila, Nanos represses apoptosis during development to ensure proper germ-line development. Unlike Nanos1 whose expression in mice is dispensable, the Nanos2 and Nanos3 proteins are required for germ cell development. Nanos2- primordial germ cells (PGCs) die only in the male gonads and show no defects in females, while Nanos3- PGCs are lost during the migration stage regardless of sex. Nanos2 and Nanos3 have distinct expression patterns during embryo development, suggesting that these two proteins do not have redundant functions. However, expression of Nanos2 can at least partially replace Nanos3 function in a Nanos3- background. Nanos3 expression can not rescue Nanos2- defects.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.