Thermo Fisher Scientific BCL-X Monoclonal Antibody (ZY214)
인간 BCL-X 단백질을 인식하는 mouse monoclonal 항체로, Western blot 및 IHC(Paraffin)에 적합합니다. 단백질 A로 정제된 액상 형태이며, 0.5 mg/mL 농도로 제공됩니다. 세포 내 핵 및 미토콘드리아 막에 발현된 BCL-X 검출에 유용합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–1 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 2–10 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | ZY214 |
| Immunogen | A synthetic peptide of human BCL-X |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS with proprietary stabilizer |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Positive control: Breast carcinoma
- Cellular location: Nucleus, mitochondria membrane
Target Information
The protein encoded by the BCL-X gene belongs to the BCL-2 protein family. Members of this family form hetero- or homodimers and act as anti- or pro-apoptotic regulators involved in various cellular activities.
These proteins are located at the outer mitochondrial membrane and regulate the mitochondrial membrane channel (VDAC) opening, which controls membrane potential, reactive oxygen species production, and cytochrome C release — all key factors in apoptosis.
Alternative splicing produces multiple transcript variants encoding two isoforms: the longer isoform functions as an apoptotic inhibitor, while the shorter isoform acts as an apoptotic activator.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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