Thermo Fisher Scientific His Tag Polyclonal Antibody
His-tag 단백질 검출용 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. Western blot, IHC, ICC, ELISA 등 다양한 응용에 적합. KLH 결합 His Tag 합성 펩타이드로 면역화. 높은 특이성과 재현성을 제공하며 연구용으로 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:400 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic peptide derived from His Tag |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 1% BSA, 50% glycerol |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
The 6x His tag is a synthetic oligopeptide consisting of six consecutive histidine residues (HHHHHH). It is commonly expressed at either the N- or C-terminal regions of recombinant proteins to allow isolation or purification by immobilized metal affinity chromatography (IMAC).
Epitope tagging enables detection of recombinant proteins lacking specific antibodies by fusing a known epitope to the protein. His-tagged proteins are purified using metal chelate chromatography due to the metal-binding properties of histidine clusters. The affinity of the His-tag motif to Ni²⁺ allows efficient purification on Ni²⁺-NTA adsorbents. His tag-specific antibodies facilitate detection and co-immunoprecipitation of tagged proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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