Thermo Fisher Scientific Ly-6E Polyclonal Antibody
마우스 반응성의 Ly-6E 폴리클로날 항체로, IHC, ICC/IF, Flow Cytometry에 사용 가능. 염소 유래 IgG 형식이며 비결합형 액상 제품. 고순도 암모늄 황산염 침전 정제. TBS(pH 7.3) + 0.5% BSA 보존, -20°C 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (IHC) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | 10 µg/mL |
| Flow Cytometry (Flow) | 10 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide with sequence SCQEKDHYCIT |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Ammonium sulfate precipitation |
| Storage Buffer | TBS, pH 7.3, with 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2933242 |
Product Specific Information
This antibody is tested in Peptide ELISA: antibody detection limit dilution 1:16,000.
Target Information
Sca1, also known as Ly6A/E, is a member of the Ly6 multigene family of type V glycophosphatidylinositol (GPI)-anchored cell surface proteins. It is expressed on multipotent hematopoietic stem cells in the bone marrow of mice with both Ly6.1 and Ly6.2 allotypes.
In the periphery, Sca1 expression varies between the two allotypes:
- Ly6.1 strains (A, BALB/c, CBA, C3H/He, DBA/1, NZB) have few Sca1⁺ resting peripheral lymphocytes.
- Ly6.2 strains (AKR, C57BL, C57BR, C57L, DBA/2, PL, SJL, SWR, 129) have relatively high numbers of Sca1⁺ lymphocytes.
Sca1 expression is dramatically upregulated in all strains upon cellular activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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