Thermo Fisher Scientific Phospho-Ask1 (Ser83) Recombinant Rabbit Monoclonal Antibody (Ask1S83-G4)
ASK1 단백질의 Ser83 인산화 형태를 특이적으로 인식하는 recombinant rabbit monoclonal 항체. Western blot 및 유세포분석에 적합하며, 높은 특이성과 재현성을 제공. 동결 보관(-20°C) 및 단백질 안정화용 buffer 포함.
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Applications
Western Blot (WB)
- Assay-dependent
- View 1 publication
Flow Cytometry (Flow)
- 1–1,000 ng/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Rat |
| Host / Isotype | Rabbit / IgG, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | Ask1S83-G4 |
| Immunogen | A synthetic phospho-peptide corresponding to residues surrounding Ser83 of human phospho Ask1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol, 0.1% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2896908 |
Additional Formats
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences from immunoreactive rabbits are cloned and expressed, followed by screening of individual clones to select optimal candidates.
Advantages include:
- Enhanced specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to the rabbit immune repertoire
Target Information
MAP3K5 (ASK1) is a serine/threonine kinase abundantly expressed in human heart and pancreas. It is activated by various extracellular stimuli such as cytokines, growth factors, and environmental stresses.
ASK1 (Apoptosis Signal-Regulating Kinase 1) activates MAPKKs (MKK4, MKK6), which in turn activate JNK and p38 MAP kinases. It plays a key role in apoptosis signaling, interacting with TRAF family members and Daxx. Overexpression of ASK1 induces apoptosis, while inactive forms inhibit TNF-α-induced apoptosis. ASK1 is expressed in various human and mouse tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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