Thermo Fisher Scientific Click-iT EdU Alexa Fluor 647 Flow Cytometry Assay Kit, 100 assays

Invitrogen™ Click-iT™ EdU Alexa Fluor™ 647 Flow Cytometry Assay Kit

The Click-iT™ EdU Alexa Fluor™ 647 Flow Cytometry Assay Kit provides a simplified and robust assay for analyzing DNA replication in proliferating cells, offering superior performance compared to traditional BrdU methods. Newly synthesized DNA is analyzed using the 633/5 nm laser of a flow cytometer.

• Accurate — Superior results compared to BrdU assays
• Fast — Results in as little as 90 minutes
• Economical — More assays per kit

View selection guide for all Click-iT™ EdU and Click-iT™ Plus EdU assays for flow cytometry

An Advanced Method Giving You Results Superior to BrdU

The most accurate method of proliferation analysis is direct measurement of DNA synthesis. Traditionally, this was achieved using radioactive nucleosides such as ³H-thymidine, later replaced by antibody-based BrdU detection. The Click-iT™ EdU Flow Cytometry Assay Kits provide a novel alternative.
EdU (5-ethynyl-2´-deoxyuridine) is a thymidine analog incorporated into DNA during active synthesis. Detection is based on click chemistry — a copper-catalyzed covalent reaction between an azide and an alkyne. In this assay, the alkyne is part of EdU, and the azide is conjugated to Alexa Fluor™ dyes (488, 647, or Pacific Blue™). Standard flow cytometry methods are then used to determine the percentage of S-phase cells.

Mild Conditions Allow Use with Cell Cycle Dyes and Antibodies

The Click-iT™ EdU labeling method uses mild conditions that preserve cell integrity and antigen recognition. The small dye azide enables efficient detection of incorporated EdU after standard aldehyde fixation and detergent permeabilization.
Unlike BrdU assays requiring harsh DNA denaturation (HCl, heat, or DNase), this EdU assay is compatible with cell cycle dyes and can be multiplexed with antibodies for surface or intracellular markers.

Quick and Simple Protocol

The Click-iT™ EdU protocol is straightforward and compatible with various fixation/permeabilization agents, including saponin and methanol.
Five simple steps to analyze your cell proliferation data:

1. Treat cells with EdU
2. Fix and permeabilize cells
3. Detect S-phase cells with Click-iT™ detection cocktail (30 min)
4. Wash once
5. Analyze by flow cytometry

Get Accurate Results Economically

By increasing the number of assays per kit, the Click-iT™ EdU Alexa Fluor™ 647 Flow Cytometry Assay Kit is more cost-effective than traditional BrdU assays, making it ideal for large-scale experiments.

For research use only. Not intended for any human or animal therapeutic or diagnostic use.

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