Thermo Fisher Scientific beta Tubulin Loading Control Monoclonal Antibody (BT7R)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000-1:5,000

View 82 publications 82 publications

Immunohistochemistry (IHC)

-

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:500

-

Immunocytochemistry (ICC/IF)

1:50-1:500

View 6 publications 6 publications

Flow Cytometry (Flow)

1 µg/test

View 1 publication 1 publication

ELISA (ELISA)

Assay-dependent

-

In vitro Assay (IV)

-

View 2 publications 2 publications

Product Specifications

Species Reactivity

Dog, Chicken, Human, Mouse, Non-human primate, Rabbit, Rat

Published species

Avian, C. elegans, Chicken, Fruit fly, Hamster, Human, Mouse, Non-human primate, Rat

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

BT7R

Immunogen

KLH conjugated peptide of the N-terminus of Beta-Tubulin. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:beta Tubulin,uniProtId:P07437-1,ncbiNodeId:9606,antigenRange:1,antigenLength:444,antigenImageFileName:MA5-16308_beta_Tubulin_P07437-1_House_mouse.svg,antigenImageFileNamePDP:MA5-16308_beta_Tubulin_P07437-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• Alexa Fluor 488
• Alexa Fluor 555
• Alexa Fluor 647
• Biotin
• DyLight 680
• DyLight 800 4X PEG
• HRP
• Request custom conjugation

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.2

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2537819

Target Information

Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. Beta-III tubulin is primarily expressed in neurons and may be involved in neurogenesis, axon guidance and maintenance. Mutations in the beta tubulin gene are the cause of congenital fibrosis of the extraocular muscles type 3. Beta-III tubulin was also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues. Glutamate residues at the C-terminus of beta III tubulin can be glutamylated. The precise function of such modifications is unclear. Tubulin is phosphorylated on Ser-172 by CDK1 during cell cycle progression. Ser-172 phosphorylation inhibits tubulin incorporation into microtubules.Microtubules, the major cytoskeletal elements found in all eukaryotic cells, consist of Tubulin, which is a dimer of two 55 kDa subunits: alpha and Beta. Microtubules play key roles in chromosome segregation in mitosis, intracellular transport, ciliary and flagellar bending, and structural support of the cytoskeleton. Because beta-tubulin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.