Thermo Fisher Scientific 8-OHdG/8 Hydroxyguanosine Polyclonal Antibody

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:400

-

Immunohistochemistry (Frozen) (IHC (F))

1:100-1:500

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

ELISA (ELISA)

1:500-1:1,000

-

Product Specifications

Species Reactivity

Chemical

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KLH conjugated 8-OhdG.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

0.01M TBS, pH 7.4, with 1% BSA, 50% glycerol

Contains

0.02% ProClin 300

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

Target Information

Oxygen is necessary for aerobic life, it can also participate in potentially toxic reactions involving oxygen free radicals and transition metals such as iron that damage membranes, proteins, and nucleic acids. n a normal human cell, there is a steady accumulation of DNA lesions with time. Substantial parts of these lesions are due to endogenous factors that damage DNA. These include reactive oxygens pecies derived from oxidative respiration. Additionally, malignant cells can produce hydrogen peroxide at levels as high as those characteristics for stimulated polymorphonuclear leukocytes. Some tumors may stimulate the defense systems of the body so that they react against the tumor to produce cytokines. Some cytokines can produce large amounts of ROS. 8-OHdG is one of the most widely studied lesions of this type. The presenceof 8-OHdG residues in DNA leads to GC to TA transversion unless repairs are made before DNA replication. Therefore, the presence of 8-OHdG may lead to mutagenesis. Furthermore, many observations indicate a direct correlation between 8-OHdG formation and carcinogenesis in vivo. It is generally accepted that the repair products of cellular DNA, for example 8-OHdG, are excreted into the urine without further metabolism. Therefore, 8-OHdG has been proposed as a urinary biomarker for in vivo oxidative DNA lesions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.