
Thermo Fisher Scientific NUCB2 Polyclonal Antibody
NUCB2 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal 항체로, Western blot 및 ELISA에 사용 가능. Human과 Rat 시료에 반응하며, 고순도 친화 크로마토그래피 정제 제품. PBS/glycerol 완충액에 보관되며 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 321–420 of human NUCB2 (NP_0050041) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 2.2 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2805067 |
Product Specific Information
Immunogen sequence:
ATEKKEFLEP DSWETLDQQQ FFTEEELKEY ENIIALQENE LKKKADELQK QKEELQRQHD QLEAQKLEYH QVIQQMEQKK LQQGIPPSGP AGELKFEPHI
Positive Samples: LO2, HeLa, Jurkat, SGC-7901
Cellular Location: Cytoplasm, Endoplasmic reticulum, Golgi apparatus, Membrane, Nucleus envelope, Peripheral membrane protein
Target Information
NUCB2 was initially identified as a calcium-binding protein with EF-hand motifs that interacts with Necdin, a growth suppressor primarily expressed in postmitotic neurons. NUCB2 is a secreted protein cleaved into three peptide products—Nesfatin-1, Nesfatin-2, and Nesfatin-3. Among these, Nesfatin-1 suppresses food intake in a leptin-independent manner. Studies suggest NUCB2/Nesfatin-1 also plays roles in energy homeostasis and neuroendocrine regulation.
Safety Information
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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