Thermo Scientific™

RPMI 1640 Medium for SILAC

Thermo Scientific™ RPMI 1640 Medium for SILAC is optimized for use with stable isotope labeling with amino acids in cell자세히 알아보기

Thermo Scientific™ RPMI 1640 Medium for SILAC is optimized for use with stable isotope labeling with amino acids in cell culture (SILAC) to analyze protein expression by mass spectrometry (MS).

Features of RPMI 1640 Medium for SILAC:
• Flexible—liquid media deficient in both L-lysine and L-arginine, allowing for more complete proteome coverage through multiple isotopic amino acid labeling
• High quality—medium is sterile, endotoxin-free, and cell culture-compatible

SILAC is a powerful method to identify and quantify relative differential changes in complex protein samples. This approach entails the in vivo metabolic incorporation of heavy 13C- or 15N-labeled amino acids into proteins followed by mass spectrometry (MS) for the accelerated and comprehensive identification, characterization, and quantitation of proteins.

This SILAC medium is used together with heavy and light amino acids for the differential isotopic labeling of cells for SILAC analysis. SILAC media supplemented with heavy amino acids are capable of nearly 100% label incorporation into proteins of living cells.

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사양

물질Cell Lysate

제품 유형Media

라벨 또는 염료Mass Isotope of Amino Acid

배송 조건Room Temperature

시작 물질Cell Lysate

최종 제품 유형Liquid Medium

수량6 x 500 mL

형태Liquid

Unit SizeEach