Thermo Fisher Scientific Nucleolin/NCL Polyclonal Antibody
Nucleolin 단백질 검출용 Rabbit Polyclonal 항체. Western blot, IHC, IP 등 다양한 응용 가능. Human에 반응하며 Rat, Hamster, Orangutan에도 예측 반응. 항원 친화 크로마토그래피로 정제된 액상 형태, 4°C 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:100–1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 525 and 575 of human Nucleolin (UniProt ID: P19338-1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | TBS, pH 7.0–8.0, with 0.1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections.
- Based on 100% sequence identity, this antibody is predicted to react with Rat, Golden hamster, and Orangutan.
Target Information
Nucleolin (NCL) is a eukaryotic nucleolar phosphoprotein involved in the synthesis and maturation of ribosomes. It is mainly located in the dense fibrillar regions of the nucleolus. The human NCL gene consists of 14 exons and 13 introns, spanning approximately 11 kb. Intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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