Thermo Fisher Scientific CD45 Monoclonal Antibody (2D1), NovaFluor Red 660, eBioscience

Thermo Fisher Scientific CD45 Monoclonal Antibody (2D1), NovaFluor Red 660, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution

• 5 µL (0.8 µg)/test

Product Specifications

Additional Formats Available

• Alexa Fluor 700
• APC
• APC-eFluor 780
• Brilliant Violet 711
• eFluor 450
• FITC
• NovaFluor Blue / Red / Violet / Yellow series
• PE, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright series
• Custom conjugation available

Product Specific Information

The 2D1 monoclonal antibody reacts with all isoforms of human CD45 (Leukocyte Common Antigen, LCA). CD45 is expressed by all hematopoietic cells except erythrocytes and platelets. The cytoplasmic portion of CD45 has tyrosine phosphatase activity and plays a key role in lymphocyte activation.

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-diluted and tested on normal human peripheral blood cells.
Use at 5 µL (0.8 µg) per test (defined as 100 µL final volume with 10⁵–10⁸ cells/test).

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., PI, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability assays.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) must be used for optimal staining.
  • Add 5 µL per sample before mixing with cells.
  • For single-color controls, use 5 µL per 100 µL of cell sample.

NovaFluor conjugates utilize Phiton™ technology with engineered fluorescent signatures optimized for minimal spillover and spectral spread.

Excitation: 637 nm
Emission: 659 nm
Laser: 633–640 nm (Red)

Target Information

CD45 (LCA) is a receptor-type protein tyrosine phosphatase expressed in all nucleated hematopoietic cells, comprising ~10% of lymphocyte surface proteins. It regulates Src-family kinases and is essential for T- and B-cell receptor signaling. CD45 isoforms arise via alternative splicing and differ in extracellular domains. It also modulates integrin-mediated signal transduction, apoptosis, and cytokine signaling.

For Research Use Only. Not for use in diagnostic procedures.

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