Thermo Fisher Scientific SIGLEC8 Polyclonal Antibody
Human SIGLEC8 단백질을 인식하는 Goat Polyclonal 항체로 Western blot에 적합. 0.5 mg/mL 농도의 액상 형태이며 TBS(pH 7.3)와 0.5% BSA 함유. -20°C 보관, 동결-해동 반복 피해야 함. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.3–1 µg/mL |
Publications
- [References not provided]
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide sequence (SYKSQLNYKTKQ) corresponding to internal amino acids of SIGLEC8 (aa 134–146) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Ammonium sulfate precipitation |
| Storage Buffer | TBS, pH 7.3, with 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_10984725 |
Product Specific Information
This antibody is tested in Peptide ELISA: antibody detection limit dilution 32,000.
Target Information
SIGLEC8 is a putative adhesion molecule that mediates sialic-acid dependent binding to cells. It preferentially binds to alpha2,3-linked sialic acid and also binds to alpha2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. SIGLEC8 is expressed specifically on eosinophils. The protein contains one copy of a cytoplasmic motif referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM), which is involved in downmodulation of cellular responses. The phosphorylated ITIM motif binds to the SH2 domain of PTPN6/SHP-1. The SIGLEC8 gene belongs to the immunoglobulin superfamily.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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