Thermo Fisher Scientific Pierce 6xHis Protein Tag Stain Reagent Set

Thermo Fisher Scientific Pierce 6xHis Protein Tag Stain Reagent Set

The Thermo Scientific™ Pierce™ 6xHis Protein Tag Stain Reagent Set is a fluorescent stain designed for preferential detection of histidine-tagged (His-tagged) recombinant fusion proteins in polyacrylamide gels (e.g., SDS-PAGE).

This unique fluorescent stain enables direct in-gel assessment of His-tagged protein expression, eliminating the need for membrane transfer and Western blotting typically used to confirm purity and yield. Detection sensitivity depends on the UV lamp (300 nm) and imaging system used (CCD camera recommended). The stain is compatible with subsequent Coomassie dye or other total protein stains.

주요 특징

• Western blot보다 2~3배 빠른 검출 속도로 연구 시간을 절약
• 젤 상 직접 검출 가능 (최소 0.2 μg의 6xHis-tagged 단백질 검출)
• Ready-to-use 2종 시약 구성으로 혼합이나 희석 불필요
• 특이적 형광 검출로 His-tagged 단백질만 선택적으로 확인 가능
• 이중 염색 호환성 – GelCode Blue Stain Reagent와 함께 사용 가능

구성품

• Stain Solution (500 mL)
• Developer Solution (500 mL)

필요 장비

• UV Lamp (300 nm)
• CCD Camera

제품 사양