Thermo Fisher Scientific PRPF8 Polyclonal Antibody
PRPF8 단백질을 인식하는 Rabbit Polyclonal 항체로, WB, IHC, IP 등 다양한 응용에 적합합니다. Human Pre-mRNA-Processing Factor 8의 특정 영역(1825–1875 aa)을 면역원으로 사용하였으며, 1 mg/mL 농도의 액상 형태로 제공됩니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution / Amount |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:500–1:2,000 |
| Immunoprecipitation (IP) | 2–10 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 1825 and 1875 of Human Pre-mRNA-Processing Factor 8 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note: For IHC, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections.
Target Information
Pre-mRNA splicing occurs in two sequential transesterification steps. The protein encoded by this gene is a component of both U2- and U12-dependent spliceosomes and is essential for the catalytic step II in the pre-mRNA splicing process. It contains several WD repeats that mediate protein-protein interactions and shows sequence similarity to the yeast Prp8 protein. This gene is a candidate for autosomal dominant retinitis pigmentosa.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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