Thermo Fisher Scientific MSK1 Polyclonal Antibody
MSK1 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody로, WB, IHC, IP 등 다양한 실험에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, TBS buffer에 보존되고 인체 및 오랑우탄에 반응성을 보입니다.
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Thermo Fisher Scientific MSK1 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:100–1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 752 and 802 of human Mitogen- and Stress-activated Protein Kinase 1 (MSK1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | TBS, pH 7.0–8.0, with 0.1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections
- Based on 100% sequence identity, this antibody is predicted to react with Orangutan
Target Information
RPS6KA5 (MSK1) is a serine/threonine protein kinase essential for the regulation of various cellular processes.
It mediates mitogen- or stress-induced phosphorylation of transcription factors such as CREB1 and ATF1, and regulates RELA, STAT3, and ETV1/ER81.
MSK1 contributes to gene activation via histone phosphorylation and plays a role in inflammatory gene regulation.
It phosphorylates CREB1 and ATF1 in response to stimuli like UV-C irradiation, EGF, and anisomycin.
MSK1 also controls RELA activity in response to TNF and glucocorticoids, interacting with the glucocorticoid receptor NR3C1 to repress inflammatory gene expression.
In skeletal myoblasts, it phosphorylates RELA at Ser-276 under oxidative stress.
In erythropoietin-stimulated cells, it mediates Ser-727 phosphorylation of STAT3 and regulates its transcriptional potential.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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