
Thermo Fisher Scientific Sheep anti-Rat IgG (H+L) Secondary Antibody
Rat IgG 검출용 Sheep 유래 Polyclonal Secondary Antibody. Western blot 및 Immunohistochemistry에 적합. 비결합(Unconjugated) 형태로 높은 특이성과 감도를 제공. 연구용으로만 사용 가능.
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Thermo Fisher Scientific Sheep anti-Rat IgG (H+L) Secondary Antibody
Applications and Tested Dilution
- Western Blot (WB): 1:2,000–1:10,000
- Immunohistochemistry (IHC): 1:1,000–1:5,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Rat |
| Host / Isotype | Sheep / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Rat IgG whole molecule |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 2 mg/mL |
| Purification | IgG fraction |
| Storage Buffer | 0.02M potassium phosphate (pH 7.2), 0.15M NaCl, 10 mg/mL BSA, 50% glycerol, 0.1 mM ZnCl₂, 1 mM MgCl₂ |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C and avoid freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_10981690 |
| Target | IgG |
| Antibody Form | Whole Antibody |
Product Specific Information
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins. They are useful for detection, sorting, or purification of rat immunoglobulins.
Secondary antibodies provide versatility for various detection systems (e.g., HRP, AP, fluorescence) and enhance sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
Typically, these antibodies are generated by immunizing the host animal with pooled immunoglobulins from the target species and may be further purified or modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) to produce highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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