Thermo Fisher Scientific BTF3L4 Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:1,000–1:3,000

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:50–1:200

Immunocytochemistry (ICC/IF)

• Tested Dilution: 1:100–1:500

Product Specifications

Product Specific Information

Antibody detects endogenous levels of total BTF3L4.

Target Information

The initiation of gene transcription involves the ordered assembly of a multiprotein complex on proximal promoter elements such as the TATA box. In addition to RNA polymerase II (Pol II), the transcription factor class II (TFII) family of proteins are required for initiation of transcription, as the first step in the formation of this initiation complex is the stable binding of TFIID to the TATA box.
An additional TFII-related protein, BTF3, does not directly associate with the proximal promoter, but rather forms a stable complex with Pol II and facilitates Pol II assembly into the complex.
The BTF3 gene is ubiquitously expressed and encodes two protein isoforms, BTF3a and BTF3b, which are produced from alternative splicing. The BTF3 proteins are identical, except that BTF3b lacks the first 44 amino acids at the N-terminal of BTF3a. As a consequence of this deletion, BTF3b is unable to induce transcription, despite being able to bind Pol II.
Additionally, BTF3a and BTF3b associate with the widely expressed protein kinase casein kinase II. Casein kinase II phosphorylates BTF3a, as well as TFIIB, and is required for the efficient transcription of the tRNA and 5S rRNA genes by Pol III.
BTF3 belongs to the NAC-beta family, which includes several related proteins, such as BTF3L1, BTF3L2, BTF3L3, and BTF3L4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

제품 이미지

(이미지 파일명: PA5-104189_BTF3L4_Q96K17-1_Rabbit.svg, PA5-104189_BTF3L4_Q96K17-1_Rabbit_PDP.jpeg)