Thermo Fisher Scientific Donkey anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody

Applications

Tested Dilution

Western Blot (WB)

Assay-dependent

Immunohistochemistry (IHC)

Assay-dependent

Immunocytochemistry (ICC/IF)

Assay-dependent

Flow Cytometry (Flow)

Assay-dependent

Immunoprecipitation (IP)

Assay-dependent

Product Specifications

Species Reactivity

Rabbit

Host/Isotype

Donkey / IgG

Class

Polyclonal

Type

Secondary Antibody

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.6

Contains

no preservative

Storage conditions

4° C

Shipping conditions

Ambient

RRID

AB_429690

Target

IgG

Cross Adsorption

Against bovine, chicken, goat, guinea pig, Syrian hamster, horse, human, mouse, rat and sheep serum proteins

Antibody Form

Whole Antibody

Product Specific Information

Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration.

Product # 31238 has been successfully used in Western blot, IF, ICC, IHC, IP and FACS applications.

Product # 31238 reacts with the heavy chains of rabbit IgG and with light chains of most rabbit immunoglobulins, but does not react against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with bovine, chicken, goat, guinea pig, Syrian hamster, horse, human, mouse, rat, and sheep serum proteins. However, this antibody may cross-react with immunoglobulins from other species.

Store product at 4°C until opened. To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C.

Country of Origin: USA

Target Information

Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.