Thermo Fisher Scientific Human PRAS40 Synthetic Peptide
Human PRAS40 합성 펩타이드로 AKT1S1의 카복시 말단 19개 아미노산 서열을 포함함. Western blot 차단용으로 사용 가능. 액상 형태, 200 µg/mL 농도, PBS 버퍼에 0.1% BSA 포함. 연구용으로만 사용.
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Applications
Control (Ctrl)
- Assay-dependent
Blocking Assay (BLOCK)
- Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Class | Synthetic |
| Type | Peptide |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Purified |
| Storage buffer | PBS, pH 7.2, with 0.1% BSA |
| Contains | 0.02% sodium azide |
| Storage conditions | -20°C |
Product Specific Information
This peptide corresponds to 19 amino acids near the carboxy terminus of human AKT1S1.
PEP-1608 can be used as a blocking peptide with polyclonal antibody PA5-34565.
For use as a blocking peptide in Western blot, incubate the peptide with equal volume of antibody for 30 min at 37ºC.
Target Information
The Akt signaling pathway contributes to the regulation of apoptosis after a variety of cell death signals.
AKT1S1, also known as PRAS40, is a proline-rich substrate of the kinase AKT1 and is thought to play a role in neuroprotection mediated by nerve growth factor (NGF) after transient focal cerebral ischemia.
AKT1S1 is also a substrate and potential regulator of mammalian target of rapamycin (mTOR), a serine/threonine kinase that regulates cell growth and cell cycle, and a negative regulator of autophagy.
Treatment with insulin-like growth factor-1 (IGF1) can induce the phosphorylation of AKT1S1 via the PI3K/AKT signaling pathway in PC12 cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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