Thermo Fisher Scientific hnRNP M1-M4 Polyclonal Antibody
Western blot, IHC, ICC/IF에 적합한 hnRNP M1-M4 폴리클로날 항체. Human, Mouse, Rat 반응성. Rabbit IgG 유래, 액상형, 1 mg/mL 농도. PBS/glycerol buffer에 보관, -20°C 저장. Affinity chromatography로 정제됨.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human HNRNPM (Accession P52272), corresponding to amino acid residues G23–A73 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2818173 |
Product Specific Information
Antibody detects endogenous levels of total hnRNP M.
Target Information
Heterogeneous nuclear ribonucleoproteins (hnRNPs) are proteins that bind directly to nascent RNA polymerase II transcripts and play a key role in transcript-specific packaging and alternative splicing of pre-mRNAs.
The M1–M4 proteins form a cluster of four abundant hnRNPs that act as pre-mRNA binding proteins in vivo and bind strongly to poly(G) and poly(U) RNA homopolymers in vitro.
These proteins belong to the ribonucleoprotein consensus sequence family and share similarity with a hypothetical RNA-binding protein from Saccharomyces cerevisiae.
They contain a unique hexapeptide-repeat region rich in methionine and arginine residues (MR repeat motif), resembling a repeat found in the 64 kDa subunit of cleavage stimulation factor involved in 3′-end maturation of pre-mRNAs.
Proteins immunologically related to M exist across diverse eukaryotes from human to yeast.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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