Thermo Fisher Scientific Ep-CAM/CD326 (Rat) Monoclonal Antibody (SPM535)
Rat Ep-CAM/CD326 단백질을 인식하는 Mouse monoclonal antibody (Clone SPM535). Western blot, ICC/IF, Flow cytometry, IP 등 다양한 응용에 적합. Protein A/G 정제, 액상 형태로 제공되며 4°C 보관. Human 시료와 반응하지 않음.
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Thermo Fisher Scientific Ep-CAM/CD326 (Rat) Monoclonal Antibody (SPM535)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:100–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:100 |
| Flow Cytometry (Flow) | 5–10 µL / 10⁶ cells |
| Immunoprecipitation (IP) | 5–10 µL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Rat |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | SPM535 |
| Immunogen | Rat small intestine cells |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | See Label |
| Purification | Protein A/G |
| Storage Buffer | Tissue culture supernatant, pH 7.4 |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Does not react with Human
Target Information
Ep-CAM (epithelial adhesion molecule, ESA) is a transmembrane glycoprotein (~40 kDa) expressed in epithelial tissues. It functions as a calcium-independent homotypic adhesion molecule and influences cell cycle, proliferation, and metabolism through activation of c-myc and cyclins A/E. Ep-CAM-mediated adhesions negatively regulate cadherin-mediated interactions, affecting epithelial differentiation and growth. Overexpression is associated with increased epithelial proliferation and is a marker for epithelial-derived tumors. Ep-CAM is found on baso-lateral surfaces of simple epithelia and many carcinoma types, distinguishing adenocarcinomas from pleural mesotheliomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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